Regarding the hormonal problems that you work with Elisa, I will mention some practical and very important tips. Be sure to use them and test yourself to understand the importance of these precious tips that have been gained through experience. Hemolysis is the leader in the TSH test, especially relative hemolysis. Not completely, it increases its value falsely. In summer, when the weather warms up, the temperature increases falsely, and in winter, the temperature decreases, resulting in a decrease in hormone values in the ELISA method. The solution: the best method is to wash the wells. If you work by hand, put it on a heater or bain-marie for a few minutes in the winter and put it in the refrigerator in the summer, and this problem will be easily solved and your controls will be 100% correct. If your washing machine is a machine, put the solution in the refrigerator for a few minutes. In the summer and in the winter, leave it in the oven and then wash it. Never use Sysmax cleaner to clean the washer because it will disturb the PH and your tests will be ruined. One of the reasons for increasing beta control is to prepare the washing solution every three days, because over time the washing solution becomes cloudy and raises the base of the tests, and blanks and other tests absorb them higher, causing an increase in false readings. and the incubation time after increasing the conjugate or after pouring the substrate A and B (dye) if it increases, it will falsely increase the test. Try to clean the entire rack containing the test tubes every time before starting to put the tests. Shake the tube several times in the English number 8, or shake each tube until it vibrates, then take the test because when the water remains still, the serum will rise and the proteins will be slightly precipitated and the hormone level will falsely decrease. For all the tests, the solution must be dissolved Diazo A and B are the same and count the total number of tests in each series and prepare a solution for all of them once, but be careful to keep the solution in a cloudy vial and avoid contamination, and this solution can be used for up to 24 hours. To avoid contamination, use a new sampler tip for each series. Try to observe the time to get the correct answer. Never remove even one standard because it will disturb your curve and you will not be able to use the removed value in the tests. read. For the beta titer, be sure to dilute the serum one to ten and compare the beta and the diluted amount and multiply the positive result by ten. For the ferritin test in thalassemia patients, dilute the serum with physiological serum one to ten and Multiply the result by ten. Dilution with distilled water has a higher reading than physiological serum and it is not good to use it for dilution if you have zero standard. If you don’t have control, you can use the standards and if you want, you can dilute the standard for example TSH 40 by one to four until it reaches about 10. That is, you can dilute the standards with zero standard or physiological serum and use it. You can also use company control to gain a good mastery in making controls.
