#Immunology
Subject: Antistreptolysin O (ASO)
Section: Practical
Qualitative method: AinRF
Tubular method 1: said
Tubular method 2:
1. We prepare two dilution tubes
First tube: 1/100, second tube: 1/500
2 ⃣ 12 tubes (can be more) removed
3. We add the first 5 tubes according to the table protocol
4⃣5, the second one as well
Tube 12 (+C), tube 11 (-C) are actually our witness tubes
5.According to the protocol in the table, we should add the amount of ASO buffer so that all the pipes except pipe 11 have a value of 1cc.
For example, before adding ASO buffer, the volume of tube number 3 was equal to 0.3, which was prepared from a 1/100 dilution tube. Now, for its volume to be 1 cc, ASO buffer must be 0. Add 7cc to it
‼ We add 11 tubes except 1.5cc with ASO
6. Add 0.5 cc of streptolysin O to all tubes except 11
‼ The difference between + and – control is specified here
7. Incubate the mixture for 15 minutes at 37 degrees
8. Add 0.5cc of 5% blood suspension to all the tubes
9. Mix the tubes separately and incubate for 45 minutes at 37 degrees, but after the first 15 minutes, mix again and centrifuge for 1-2 minutes at 1500 rpm.
Observation of hemolyses. The last tube that is not hemolyzed becomes our Tod’s dilution
‼+ control: definitely has hemolysis and negative control is the opposite
@Probe_Lab
This post is written by Amer_7798