Anti Thyroid Peroxidase Antibody test
Sampling considerations:
According to the instructions of the laboratory, a venous blood sample should be taken from the patient.
Check the blood sampling site for bleeding.
The blood sample must be centrifuged within two hours and the serum separated.
The separated serum should be clear and free of fibrin, hemolysis or lipemia.
If you use thyroid-related drugs, write it down in the test sheet.
The sampler should ask about the patient’s clinical condition and write it down in the test sheet if necessary.
Maintenance conditions:
The sample is stable for 7 days at 2-8oC and 30 days at -20oC. Preferably, it should be kept at 2-8oC until the time of testing. If the test takes too long, freeze it.
Clinical applications:
Help in the diagnosis of autoimmune thyroid diseases such as Hashimoto’s thyroiditis and Graves’ disease.
Differentiating thyroid autoimmune diseases from non-autoimmune goiter or hypothyroidism.
As a diagnostic method in deciding to treat a patient with subclinical hypothyroidism.
Interpretation:
In hypothyroid patients, the increase of Anti-TPO with 95% sensitivity supports the diagnosis of autoimmune thyroiditis. Although the increase in the level of anti-thyroid peroxidase antibodies (Anti-TPO) is observed in many types of thyroid diseases, the most common is chronic thyroiditis (Hashimoto’s thyroiditis in adults and lymphocytic thyroiditis in children). This antibody is present in 70-90% of patients with Hashimoto’s thyroiditis. Chronic thyroiditis is related to other autoimmune diseases (vascular collagen).
Anti-TPO test is also observed in other autoimmune diseases such as diabetes mellitus type 1, rheumatoid arthritis and pernicious anemia. Anti-TPO may be positive in 12% of women and 1% of healthy and asymptomatic men. This test is often performed along with the anti-thyroglobulin (Anti-TG) antibody test to increase its sensitivity and specificity.
Intervening factors:
Immunosuppressive drugs may affect the results.
About 10% of adults (especially women) have low titers of Anti-TPO without any other evidence of thyroid disease.
In some people who are exposed to animal environments (for example in animal clinics), they may have antibodies against animal antigens. These antibodies may cross-react with thyroid microsomal antibodies and lead to invalid results.
Description:
Anti-TPO test is mainly used to differentiate thyroid diseases.
90% of autoimmune thyroiditis patients have anti-peroxidase thyroid antibodies. Anti-thyroglobin antibody (Anti-TG) is also found in most patients.
Often, this test is performed with anti-thyroglobulin antibody to increase its sensitivity and specificity.
These antibodies can be transferred from mother to fetus if they have a high level in the third trimester of pregnancy and may cause thyroid disorders.
This post is written by jm30303030