#Serology
#Miscellaneous
How to use qualitative, semi-quantitative and even quantitative methods?!!!
For example, there is no difference for CRP, for example, I will say this test
1⃣ Qualitative method: the same slide
We want 3 circular slides
1) negative control (-C)
2) Positive control (+C)
3) My head
Working method:
Negative control + antigen aglot 100%, we don’t see it because it is negative (that’s why they call it negative because we already know that we don’t have aglot)
We see positive control + antigen aglot 100% (that’s why they call it positive because we already know it has aglot)
Serum + antigen??? Must check
If no agglutination occurs, the test is negative
_If agglutination occurs, the answer is positive, but we must determine its amount
Agglut with large grains 3+P (positive and intense)
Agglut with seeds medium 2+P (positive and medium)
Agglut with small grains 1+P (positive and weak)
Now we go to the semi-quantitative method for more confirmation and to increase the accuracy of the work and a better answer
2. Semi-quantitative method: the same slide is slightly different, what is the difference?
80 microns once, 40 microns once, 20 microns once, 10 microns once and finally 5 microns
Working method:
We add 80 microns of the serum to the tile (the same circular slide) and write a dilution of 1/10 on it.
We add 40 micrograms of serum to the tile and write 1/20 dilution above it
We add 20 micrograms of serum to the tile and write 1/40 dilution above it
Add 10 micrograms of serum to the tile and write 1/80 dilution above it
So far, 4 slides have been filled with different dilutions
We add antigen to each slide and check the agglutination
And the first slide where aglut does not occur is Tad Ma
3.Quantitative method: We prepare the same semi-quantitative but more basic dilution, that is, we also use physiological serum
Working method:
With the help of physiological serum, we prepare a dilution of 1/10 (0.1 cc of serum with 0.9 cc of physiological serum) and prepare a serial dilution of 1/2 (that is, I take 0.5 cc of physiological serum and 0.5 cc of the patient’s serum from the first tube and the second tube Third, I take 0.5 cc of physiological serum with 0.5 cc of the patient’s serum from tube two to the end)
First tube 1/10
Double tube 1/20
For example, 10 and then we will out it
The more the number of tubes, the better, but at least 11 tubes is good (11 control tubes or the same control).
Add antigen to each tube and incubate
Viewing the aglot, the first tube where aglot did not occur becomes our tod (diluted photo, for example, tube number 7, which becomes 1/720, becomes our tod 720)
So how it works: in order of greater accuracy
Kifinime kimikami
little quality
semi-comic
@Probe_Lab
This post is written by Amer_7798