Nano technology helps diabetic patients!
Subject: science and research #insulin #biosensor #gold_nanoparticles
News date: 06/19/2016
http://ars.els-cdn.com/content/image/1-s2.0-S092777651730499X-fx1_lrg.jpg
The researchers of Isfahan University of Technology have succeeded in making a biosensor laboratory sample that can determine the amount of blood insulin without the need for sample preparation with high sensitivity and accuracy. Gold nanoparticles have been used in the construction of this biosensor.
Diabetes is one of the most common diseases that, if not paid enough attention to, has irreparable risks for the patient. According to global statistics, every 10 seconds two people in the world are diagnosed with diabetes and every 8 seconds one person dies. The promising thing about this disease is that it can be easily controlled by controlling the amount of insulin.
Professor Ali Asghar Insafi, a member of the faculty of Isfahan University of Technology, introduced the purpose of this research to make a sensitive and selective biosensor (biosensor) for measuring insulin and said: “The high selectivity of this biosensor without the need for special operations on the target sample and “Preparation is one of its most important features.”
Insulin is one of the most important hormones secreted in the body, which, in addition to controlling blood sugar, plays an essential role in many metabolic activities of the body. Therefore, measuring and controlling the amount of insulin in healthy and sick people plays a prominent role in controlling diabetes.
About the capabilities of the designed sensor, Essafi said: “This biosensor is able to measure the amount of insulin in the range of 1 to 1000 nmol/liter with high accuracy. High sensitivity and selectivity are other advantages of this sensor. Therefore, its use in medical diagnosis laboratories will be very useful.
According to Insafi, in the construction of this biosensor, polyorthophenylene diamine polymer was placed electrochemically on the surface of gold nanoparticles, which were placed on the substrate of pencil brain. Then, the specific insulin-sensitive single-stranded DNA has been attached to the above-mentioned substrate through a sulfide bond.
Studies have shown that in the presence of magnesium ion, insulin creates a tetrahedral cage after interacting with the DNA chain. This structure change will be used as a tool to detect insulin in small amounts by electrochemical impedance method.
At the end, Essafi explained the reason for using gold nanoparticles and the importance of their presence in sensor manufacturing as follows: “Designing and manufacturing biosensors based on DNA requires a method that can firstly maintain DNA stably in the sensor substrate and secondly, the number of DNA chains is placed He controlled the bed. These two important issues can be controlled by using gold nanoparticles modified with other materials, and without the presence of modified nanowires, it will not be possible to control and stabilize the sensor.”
This research is the result of the efforts of Professor Ali Asghar Ensafi and Professor Behzad Rezaei – faculty members of Isfahan University of Technology – and Elaha Khodami, PhD student of Analytical Chemistry at Isfahan University. The results of this work have been published in the journal Colloids and Surfaces B: Biointerfaces with an impact factor of 887.3 (Vol. 159, 2017, pages 47-53).
Nanobiotechnology Laboratory
Comprehensive Specialized Center for the Synthesis of Plasmonic Nanoparticles (Gold and Silver)
https://t.me/NanobiotechLab
.
To contact and order the product:
@NanoBioLab
021-8288 4756
Source:
http://www.sciencedirect.com/science/article/pii/S092777651730499X?via%3Dihub
This post is written by NanoBioLab